2P Calcium Imaging
Event Detection Parameter Explorer
d′detectability index (≥3 reliable)
ΔF/Findicator's fractional response per AP
F₀baseline photon flux (photon/s)
τdecay time constant
Readout
\(d'\)
F₀ at setup
Line spacing Y
Pixel size X
FOV
Frame rate
Nyquist
Pulses/µm (center)
Pulses/px (center)
Neuron diameter (µm)
Pulses across neuron
Neuron width
Indicator
ΔF/F
τ
Flux Calibration
F₀ measured at reference FOV.
F₀ (photon/s per cell)
at FOV area (mm²)
Photon flux depends on the properties of the imaging system, the sample, laser power and indicator.
FOV Dimensions
FOV X (µm)
FOV Y (µm)
Scan Rate
Lines / s (resonant)
Lines / frame (Y res)
Frame rate
Laser
Pulse rate (MHz)
